Question

Sodium dihydrogen phosphate NaH2PO4 (monobasic) and sodium hydrogen phosphate Na2HPO4 (dibasic) are a weak acid and its conjugate base pair that are mixed to make a buffer with pH 7.2. The pKa of the acid-base transition below is 7.2. Use the Henderson-Hasselbalch equation below: pH = pKa + log ([A-])/([HA]). a. Determine the molar ratio of NaH2PO4 and Na2HPO4 in the buffer with pH = 7.2. What would be the concentrations of NaH2PO4 and Na2HPO4 in a 20 mM buffer with pH = 7.2? b. Calculate the amount of monobasic and dibasic solids to prepare 20 mL of a 20 mM buffer, pH = 7.2 (use the molecular weights of NaH2PO4 and Na2HPO4 to determine how much to weigh out).

          Sodium dihydrogen phosphate NaH2PO4 (monobasic) and sodium hydrogen phosphate Na2HPO4 (dibasic) are a weak acid and its conjugate base pair that are mixed to make a buffer with pH 7.2. The pKa of the acid-base transition below is 7.2. Use the Henderson-Hasselbalch equation below: pH = pKa + log ([A-])/([HA]).

a. Determine the molar ratio of NaH2PO4 and Na2HPO4 in the buffer with pH = 7.2. What would be the concentrations of NaH2PO4 and Na2HPO4 in a 20 mM buffer with pH = 7.2?

b. Calculate the amount of monobasic and dibasic solids to prepare 20 mL of a 20 mM buffer, pH = 7.2 (use the molecular weights of NaH2PO4 and Na2HPO4 to determine how much to weigh out).

Added by Alejandro H.

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