00:02
Okay, the question is, what are the principles of the sds, jels, electrophosis? which characteristics of the proteins are very used to separate them and were you able to identify the protein of your interest? okay, which test did you use, identify the proteins? mention the number of the fractions we tested the sds pages, electrophosis, and explained why.
00:30
And the last one is, what is the approximates molecular rate of the studies, proteins, and proteins, and proteins.
00:35
Is it close to expected the glycosides? okay, the principles of sds pages state that charged molecules migrated to the electrodes with the opposite signs when placed an electric field, separations of the charge molecules depend upon the relative mobility of the charged species.
01:00
The smaller the molecules migrated faster due to the less resistance during the electrolysis, electropholids.
01:07
So what property used to separate the protein, chromotography can be used to separate the protein in a solution or under the de -natureing the conditions by using the porous gels.
01:18
So this technique also known as a size excretion to chromatography, the principle is that the smaller molecules have to transfer a larger volume in the porous matrix...