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Hi, in this question we need to describe about hplc and mass spectroscopy, their principles and their applications.
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Hplc is abbreviated as high performance liquid chromatography.
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It is an analytical technique that is used to separate, identify and quantify compounds in a mixture.
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Mass spectroscopy, it is the analytical chemistry technique that helps to identify the amount and type of chemicals present in the mixture by measuring mass to charge ratio and abundance of gas phase ions.
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Now their principle, hplc works on the principle that when the compounds are introduced in hplc column, they travel according to their relative affinities towards the stationary phase.
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The compound which are more affinity towards the stationary phase travels slower and vice versa.
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Mass spectroscopy principle that it generates multiple ions from the sample under investigation.
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The molecular ion undergoes fragmentation...