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2. Calculate isoelectric point (pI) of following peptide. Gly-Glu-Ala-Lys-Tyr-Arg-Leu pKa N terminal NH3+ 9.6 Glu 4.25 Lys 10.53 Arg 12.48 C terminal COOH 2.36 3. Protein X has a 100 nM affinity for ligand Q. How much of ligand Q is required for the following fractions of protein X to exist in the bound state? a) 0.10 b) 0.30 c) 0.50 d) 0.90 Protein Z also binds ligand Q. At a concentration 200 mM Q, of protein Z is 70% bound. Which protein (X or Z) has a greater affinity for ligand Q?

          2. Calculate isoelectric point (pI) of following peptide. Gly-Glu-Ala-Lys-Tyr-Arg-Leu pKa N terminal NH3+ 9.6 Glu 4.25 Lys 10.53 Arg 12.48 C terminal COOH 2.36 3. Protein X has a 100 nM affinity for ligand Q. How much of ligand Q is required for the following fractions of protein X to exist in the bound state? a) 0.10 b) 0.30 c) 0.50 d) 0.90 Protein Z also binds ligand Q. At a concentration 200 mM Q, of protein Z is 70% bound. Which protein (X or Z) has a greater affinity for ligand Q?

$2. Calculate isoelectric point (pI) of following peptide. Gly-Glu-Ala-Lys-Tyr-Arg-Leu pKa N terminal NH3+ 9.6 Glu 4.25 Lys 10.53 Arg 12.48 C terminal COOH 2.36 3. Protein X has a 100 nM affinity for ligand Q. How much of ligand Q is required for the following fractions of protein X to exist in the bound state? a) 0.10 b) 0.30 c) 0.50 d) 0.90 Protein Z also binds ligand Q. At a concentration 200 mM Q, of protein Z is 70% bound. Which protein (X or Z) has a greater affinity for ligand Q?$

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