2. Determine the theoretical oxygen demand for the amino acids glycine and leucine using the formula discussed here. Theoretical oxygen demand (ThOD) for nutrient source (CcHhOoNn) is calculated by the below equation: c + (h - 3n)/4 - o/2 = ThOD [mol O2/mol substrate]. Convert to g O2 per g substrate by multiplying by the molar mass of the substrate (in this case, glycine and leucine) and dividing by the molar mass of O2 (32 g/mol). Calculate the ThOD in units of g O2 per g substrate for both glycine and leucine.
3. Based on your answer to Q2 and the constraints mentioned above, would wastewater feed containing either only glycine or only leucine need nitrogen supplement? Assume a basis of 100 g oxygen demand of amino acid.