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2) Rosemary crude extract has been analyzed by HPLC device which has reversed-phase packing column. The chromatogram of crude extract is given below. Each peak belongs to different metabolites as described. Using this chromatogram, a) Determine the retention time of each metabolite, b) Sort the components according to their increased polarity, c) Please explain the resolution performance of carnosol and carnosic acid for this separation. Figure 3. The HPLC-UV chromatogram of rosemary crude extract - 1, carnosic acid; 2, carnosol; 3, rosmanol isomer; 4, cirsimaritin; 5 (epi)rosmanol methylester; 6 , rosemadial; 7, apigenin; \( 8, \mathrm{C}_{19} \mathrm{H}_{26} \mathrm{O}_{3} ; 9 \), methyl carnosate

          2) Rosemary crude extract has been analyzed by HPLC device which has reversed-phase packing column. The chromatogram of crude extract is given below. Each peak belongs to different metabolites as described. Using this chromatogram,
a) Determine the retention time of each metabolite,
b) Sort the components according to their increased polarity,
c) Please explain the resolution performance of carnosol and carnosic acid for this separation.

Figure 3. The HPLC-UV chromatogram of rosemary crude extract - 1, carnosic acid; 2, carnosol; 3, rosmanol isomer; 4, cirsimaritin; 5 (epi)rosmanol methylester; 6 , rosemadial; 7, apigenin; \( 8, \mathrm{C}_{19} \mathrm{H}_{26} \mathrm{O}_{3} ; 9 \), methyl carnosate

2) Rosemary crude extract has been analyzed by HPLC device which has reversed-phase packing column. The chromatogram of crude extract is given below. Each peak belongs to different metabolites as described. Using this chromatogram,
a) Determine the retention time of each metabolite,
b) Sort the components according to their increased polarity,
c) Please explain the resolution performance of carnosol and carnosic acid for this separation.

Figure 3. The HPLC-UV chromatogram of rosemary crude extract - 1, carnosic acid; 2, carnosol; 3, rosmanol isomer; 4, cirsimaritin; 5 (epi)rosmanol methylester; 6 , rosemadial; 7, apigenin; 8, C19H26O3 ; 9, methyl carnosate

Added by Marc C.

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