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2.5 : Minimal growth medium for bacteria such as E. coli includes various salts with characteristic concentrations in the mM range and a carbon source. The carbon source is typically glucose and it is used at 0.5% (a concentration of 0.5 g/100 mL). For nitrogen, minimal medium contains ammonium chloride (NH4Cl) with a concentration of 0.1 g/100 mL. (a) Make an estimate of the number of carbon atoms it takes to make up the macromolecular contents of a bacterium such as E. coli. Similarly, make an estimate of the number of nitrogens it takes to make up the macromolecular contents of a bacterium? What about phosphate? (b) How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the carbon? How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the nitrogen? Note that this estimate will be flawed because it neglects the energy cost of synthesizing the macromolecules of the cell. These shortcomings will be addressed in Chapter 5.

          2.5 : Minimal growth medium for bacteria such as E. coli includes various salts with characteristic concentrations in the mM range and a carbon source. The carbon source is typically glucose and it is used at 0.5% (a concentration of 0.5 g/100 mL). For nitrogen, minimal medium contains ammonium chloride (NH4Cl) with a concentration of 0.1 g/100 mL.
(a) Make an estimate of the number of carbon atoms it takes to make up the macromolecular contents of a bacterium such as E. coli. Similarly, make an estimate of the number of nitrogens it takes to make up the macromolecular contents of a bacterium? What about phosphate?
(b) How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the carbon? How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the nitrogen? Note that this estimate will be flawed because it neglects the energy cost of synthesizing the macromolecules of the cell. These shortcomings will be addressed in Chapter 5.

2.5 : Minimal growth medium for bacteria such as E. coli includes various salts with characteristic concentrations in the mM range and a carbon source. The carbon source is typically glucose and it is used at 0.5% (a concentration of 0.5 g/100 mL). For nitrogen, minimal medium contains ammonium chloride (NH4Cl) with a concentration of 0.1 g/100 mL.
(a) Make an estimate of the number of carbon atoms it takes to make up the macromolecular contents of a bacterium such as E. coli. Similarly, make an estimate of the number of nitrogens it takes to make up the macromolecular contents of a bacterium? What about phosphate?
(b) How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the carbon? How many cells can be grown in a 5 mL culture using minimal medium before the medium exhausts the nitrogen? Note that this estimate will be flawed because it neglects the energy cost of synthesizing the macromolecules of the cell. These shortcomings will be addressed in Chapter 5.

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