The triprotic acid, phosphoric acid, dissociates in the following steps:
H3PO4 -> H2PO4- + H+
H2PO4- -> HPO42- + H+
HPO42- -> PO43- + H+
Given that you have stock solutions of NaH2PO4 and Na2HPO4, calculate how many mL of each solution you will need to combine to prepare 100 mL of a 100 mM solution of phosphate buffer pH 7.0.
Given that the molecular weight of the amino acid histidine is 155.15 g/mol, calculate how many grams of histidine should be dissolved in 10 mL of the 100 mM phosphate buffer to yield a 1 mM solution of histidine in phosphate buffer.