9. ATP formation from ADP and H2PO4- is nonspontaneous process. This reaction is connected with creatine-phosphate hydrolysis which has negative ΔG° value:
ADP + H2PO4- → ATP + H2O; ΔG° = +30 kJ
Creatine-phosphate + H2O → creatine + phosphate; ΔG° = -43 kJ
Use principle of thermodynamic conjugation to prove the possibility of spontaneous process in the body: Creatine-phosphate + ADP → Creatine + ATP