Question

A biologist needs 500 mL of a 10 mM phosphate buffer. The stock solution of the buffer is 100 mM. How much stock solution should be used to make the 10 mM phosphate buffer?

Name: a biologist needs 500 ml of a 10 mm phosphate buffer the stock solution of the buffer is 100mm how much stock soln should be used to make the 10 mm phosphate buffer 83872
Uploaded: 2021-10-18T18:10:08-08:00
Duration: 3 min 13 s
Channel: Jennifer Hudspeth
Description: a biologist needs 500 ml of a 10 mm phosphate buffer the stock solution of the buffer is 100mm how much stock soln should be used to make the 10 mm phosphate buffer 83872

          A biologist needs 500 mL of a 10 mM phosphate buffer. The stock solution of the buffer is 100 mM. How much stock solution should be used to make the 10 mM phosphate buffer?

Added by Patrick C.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Jennifer Hudspeth

10/18/2021

Step 1

Step 1:** Calculate the volume of stock solution needed using the dilution formula: M1V1 = M2V2 Given: M1 = 100 mM V1 = volume of stock solution to be used (unknown) M2 = 10 mM V2 = 500 mL Plugging in the values: 100 mM * V1 = 10 mM * 500 mL ** Show more…

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A biologist needs 500 mL of a 10 mM phosphate buffer. The stock solution of the buffer is 100 mM. How much stock solution should be used to make the 10 mM phosphate buffer?