Question

A new translation. A transfer RNA with a UGU anticodon is enzymatically conjugated to $^{14} \mathrm{C}$ -labeled cysteine. The cysteine unit is then chemically modified to alanine. The altered aminoacyl-tRNA is added to a protein-synthesizing system containing normal components except for this tRNA. The mRNA added to this mixture contains the following sequence: $$5^{\prime}-UUUUGCCAUGUUUGUGCU-3'$$ What is the sequence of the corresponding radiolabeled peptide?

          A new translation. A transfer RNA with a UGU anticodon is enzymatically conjugated to $^{14} \mathrm{C}$ -labeled cysteine. The cysteine unit is then chemically modified to alanine. The altered aminoacyl-tRNA is added to a protein-synthesizing system containing normal components except for this tRNA. The mRNA added to this mixture contains the following sequence:
$$5^{\prime}-UUUUGCCAUGUUUGUGCU-3'$$
What is the sequence of the corresponding radiolabeled peptide?

Added by Jeffrey R.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Adi S

07/07/2023

Step 1

The start codon is typically AUG, which codes for methionine (Met). Given mRNA sequence: \[ 5' - UUUUGCCAUGUUUGUGCU - 3' \] The start codon AUG is located at positions 6-8. Show more…

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A new translation. A transfer RNA with a UGU anticodon is enzymatically conjugated to $^{14} \mathrm{C}$ -labeled cysteine. The cysteine unit is then chemically modified to alanine. The altered aminoacyl-tRNA is added to a protein-synthesizing system containing normal components except for this tRNA. The mRNA added to this mixture contains the following sequence: $$5^{\prime}-UUUUGCCAUGUUUGUGCU-3'$$ What is the sequence of the corresponding radiolabeled peptide?