After measuring the absorbance vs concentration (and working with the same spectrophotometer), you realized you had worked with the wrong λmax. Which of the following will get affected in the regression line for the calibration curve?
- Concentration
- Slope of the absorbance vs concentration plot
- Length of the spectrophotometer
- Transmittance
Why do we only record absorbance at a wavelength that gives the greatest absorbance value for Allura Red (λmax)?
A solution absorbs 50% of a specific wavelength of light that passes through it in a spectrophotometer. What is the % transmittance and absorbance at this wavelength for this solution? (Hint: use Absorbance = -log(I/Io) and (I/Io) * 100% = % Transmittance)
After you finish collecting your absorbance readings on the spectrophotometer, you notice a dark smudge on your cuvette that you used to blank while doing a Beer's Law experiment. How would you expect this to have affected your absorbance readings on samples you ran after the blank?