00:01
Hey everyone.
00:02
In this lesson will determine the sequence of an octopeptide using information from hydrolysis, chemical cleavage, and enzymatic digestion.
00:10
We'll analyze the amino acid composition, identify the n terminal residue, and use cyanogen bromide and trips and digestion data to piece together the full sequence.
00:35
Identifying the n terminal residue, the peptide was treated with fdnb, m2, 4, dinitrol phenololololene, which is a dmp, ala, was detected.
01:10
So this means ala is the n -terminal amino acid.
01:30
Cyanogen bromide, cleavage analysis.
01:48
Cnbr cleaves at mesheoin, mesheloin, which is m, resulting in two fragments.
01:59
So we have a pentapeptide containing two lysine residues.
02:25
And we have a tripeptide containing phall, gli, and alla.
03:01
Tripsin digestion analysis.
03:10
Tripsin cleaves at arginine r and lysine, generating three fragments.
03:22
We have a dipeptide, a four residue fragment in gav...