00:01
Hello students, we have asked a question, what is the disadvantage of roller bottle culture of anchorage dependent animal cell culture? show the different reactor configuration for overcoming this problem and discuss in brief about reactors.
00:21
The answer for this question is the disadvantage of roller bottle culture of anchorage dependent animal cell culture is that, it limits the number of cells that can be grown in a given volume the main advantage disadvantage is that it limits the number of cells that can be grown in given volume this is because the cells because the cells are attached attached to the vaults of the bottle.
02:00
So the total number, so the total number of cells is limited by the surface area of that bottle.
02:38
Now to overcome this problem, overcome this problem, several reactors.
03:00
Configurations have been developed such as a rocker bottle second is a airlift reactor and third one is steed tank reactor the rocker bottle is designed to increase the surface area of the bottle allowing more more cells to be grown in the same volume to provide more surface area.
04:24
The airlift reactor is a cylindrical vessel with a baffles that create an upward flow of the air allowing the cells to be evenly distributed throughout the vessel...