Answer question in image
Absorbance (595 nm) values obtained from a number of BSA standard solutions.
Table 3. Absorbance values (595 nm) of BSA standard solutions
BSA concentration/mg/ml Absorbance (1st repeat) Absorbance (2nd repeat)
0 0 0
0.2 0.219 0.208
0.4 0.298 0.302
0.6 0.387 0.395
0.8 0.483 0.476
1.0 0.587 0.599
1.2 0.701 0.712
Answer the following questions (each 1 mark), giving answers closest to the selection available:
(a) Plot an appropriate calibration graph using Excel; what is the equation of best fit?
(b) Following on from (a), what is your R2 value?
(c) Using your plot used to answer (a), calculate the mean and standard deviation of the unknown BSA concentration if replicate absorbance values were 0.591, 0.596, and 0.588.
y = 0.6021x + 0.0212
y = -0.5121 - 0.0211
y = -0.5079 + 0.0295
y = 0.5621x - 0.0412
y = 0.5461x + 0.0557
0.9732
0.9810
0.9401
0.9901
0.9312
0.98 +/- 0.01 mg/ml
0.91 +/- 0.03 mg/ml
0.89 +/- 0.01 mg/ml
0.93 +/- 0.04 mg/ml
0.82 +/- 0.09 mg/ml