00:01
Briefly explain how to prepare aga.
00:02
So aga is the unit of medium for bacteria or some other microorganism to grow.
00:09
So the main step will be first of all you want to measure the ingredient.
00:26
Typically about 1 .5 % aga powder.
00:38
That is 1 .5 gram per 100 milliliter of water.
00:46
And also sometimes you have a nutrient.
00:49
Medium like nutrient broth.
01:04
So once you weigh them out, you're going to mix these ingredients with water.
01:22
So in a heat -proof container you're going to have to combine the powder with distilled water and then stir the mixture, make sure that the aga is evenly distributed, and then heat the mixture on a stuff top top and stir frequently until the augur completely dissolve.
02:27
Now, if necessary, you have to sterilize aga solution by autoclave or boil it until it's free from contaminants, but usually it's autoclave...