Calculate the standard free energy change for the reduction of O2 by NADH. The relevant standard reduction potentials for the two half-reactions are:
E°' (V)
½ O2 + 2 H+ + 2 e- → H2O
0.815
NAD+ + H+ + 2 e- → NADH
-0.315
The Faraday constant is 96,485 J*V-1*mol-1.
Extra Credit: Is the reaction spontaneous? Where in an organism does this reaction occur?
(Hint: this reaction does not occur in a single step.)