CALCULATIONS FOR THE UNKNOWN AMINO ACID TITRATION 1. Plot your titration curves (using Excel) and locate the end points which are the regions of steepest slope. If you have trouble locating the end points, remember that the two end points should be equally spaced along the x-axis. 2. Deduce approximate pKa values by observing the pH in the buffer regions half way between the end points where pH = pKa.
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First, plot the titration curve using Excel. To do this, you need to have the data points for the volume of titrant added (X-axis) and the corresponding pH values (Y-axis). Show more…
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