Question

CHEM 255 Spinach Extraction Lab Name Isolation of Chlorophyll and Carotenoid Pigments from Spinach Post-Lab Questions 1. How would using a more polar solvent affect the equilibrium between solid and solution for a very polar compound? 2. What would happen to the Rf values of the pigments if the relative concentration of acetone in the developing solution was increased? Briefly explain. 3. Why are the chlorophylls less mobile than the carotenes on the TLC plate? Which compound is most polar and which is least polar? How do you know? 4. Identify as many spots on the TLC plate as you can. Determine which pigments were present in the yellow band and which were present in the green band from your column. Draw their structures.

          CHEM 255
Spinach Extraction Lab
Name
Isolation of Chlorophyll and Carotenoid Pigments from Spinach

Post-Lab Questions
1. How would using a more polar solvent affect the equilibrium between solid and solution for a very polar compound?
2. What would happen to the Rf values of the pigments if the relative concentration of acetone in the developing solution was increased? Briefly explain.
3. Why are the chlorophylls less mobile than the carotenes on the TLC plate? Which compound is most polar and which is least polar? How do you know?
4. Identify as many spots on the TLC plate as you can. Determine which pigments were present in the yellow band and which were present in the green band from your column. Draw their structures.

CHEM 255
Spinach Extraction Lab
Name
Isolation of Chlorophyll and Carotenoid Pigments from Spinach

Post-Lab Questions
1. How would using a more polar solvent affect the equilibrium between solid and solution for a very polar compound?
2. What would happen to the Rf values of the pigments if the relative concentration of acetone in the developing solution was increased? Briefly explain.
3. Why are the chlorophylls less mobile than the carotenes on the TLC plate? Which compound is most polar and which is least polar? How do you know?
4. Identify as many spots on the TLC plate as you can. Determine which pigments were present in the yellow band and which were present in the green band from your column. Draw their structures.

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