00:01
By using the law of beer -lambert, we know that a that is absorbent is equal to e into c into l.
00:22
In this equation, a stands for absorbance.
00:30
Calculate equal to polar absorvity.
00:41
Polar absorvity which is multiplied with c which is the concentration, the compounds and l which is the path length of the cuvette.
01:00
This is the equation for beer -lambert's law.
01:04
Now we have the given value upon which we have to calculate the concentration.
01:13
So first we have to calculate the concentration or compound a at specific spectrum.
01:23
When compound a is at 485 nm, concentration of a1 is equal to, let's say a1 is equal to 0 .533 divided by 3 .55 into 10 to the power 10 multiplied with 1.
01:53
The value is equal to 1 .50 into 10 to the power minus 4 per liter.
02:01
Similarly, at the nanometer of 640 nm, the concentration of compound a, let's say it as a2 is equal to 0 .590 divided by 0 .64 into 10 to the power 2 multiplied with 1.
02:34
The value is equal to, the value of a2 is equal to 1 .04 into 10 to the power minus 3.
02:48
Now we have to calculate the concentration b...