DATA FOR STANDARD CURVE
Measure the migration distance to the center of the protein band of interest. An image of the protein molecular weight markers will be posted on HuskyCT. Note: 250 kilodaltons = 250,000 daltons = 250,000g/mole
Table 12.1
PROTEIN BAND | MOLECULAR WEIGHT (kilodaltons) | MIGRATION DISTANCE (cm) | Rf
TRACKING DYE FRONT | - | 7.2cm | 1
MARKER | 250 | 1.8cm | 0.25
MARKER | 150 | 2.1cm | 0.29
MARKER | 100 | 3.4cm | 0.472
MARKER | 75 | 3.7cm | 0.5138
MARKER | 50 | 4.6cm | 0.6388
MARKER | 37 | 5.3cm | 0.7361
MARKER | 25 | 5.9cm | 0.8194
MARKER | 20 | 6.2cm | 0.861
MARKER | 15 | 6.5cm | 0.902
MARKER | 10 | 6.6cm | 0.916
PROMINENT PROTEIN BAND #1 (ELUTED WITH 25mM IMIDAZOLE) | 41.5KDa | |
PROMINENT PROTEIN BAND #2 (ELUTED WITH 250mM IMIDAZOLE) | 40.5KDa | |
PROMINENT PROTEIN BAND #1 (ELUTED WITH 400mM IMIDAZOLE) | 40 KDa | |