Question

DENATURING AGENTS: HOW TO SHIFT THE PROTEIN UNFOLDING EQUILIBRIUM TO THE RIGHT (5 pts) Which of the following statements about the equilibrium “m” value, for the unfolding of a biomolecule in the presence of a denaturing agent, is FALSE? In your homework sheet, provide an answer to this question by simply writing a, b, c, d or e. (a) The absolute value of the “m” value is proportional to the amount of solvent-accessible surface is very different from the conformation of the heat-unfolded  repressor. (c) Cold unfolding has a Cp of zero. (d) Cold-unfolding is characterized by a negative H0 and S0. It is likely that the value of S0 is negative mostly due to the reduced dynamics of the waters surrounding the cold-unfolded protein. (e) Cold unfolding has a negative Cp. 6. DETERMINING HEAT CAPACITY CHANGES UPON PROTEIN UNFOLDING: DIFFERENTIAL SCANNING CALORIMETRY (DSC). (3 + 2 = 5 pts) a. Draw the complete DSC plot (Cp vs T) for the equilibrium unfolding of a protein that is sufficiently unstable so that both its cold- and heat-induced unfolding transitions can be observed in liquid solution by DSC. Be sure to draw lines, curves and areas with a biologically meaningful sign, based on what you know about protein folding and unfolding at equilibrium. Label Tm, Tm’, Cp,heat-unfolding and Cp,cold-unfolding on the plot. b. Draw the DSC profile for the order-disorder transition of a nonpolar lipid with Cp = 0. - 3 - area that becomes exposed, upon denaturant-induced protein unfolding. (b) Proteins with a large and negative “m” value unfold easily in the presence of a denaturing agent. (c) At the midpoint of a denaturant-induced equilibrium unfolding curve the “m” value switches sign. (d) The “m” value is usually negative in the unfolding direction and positive in the folding direction. The absolute value of “m” is the same in both directions. (e) The unfoldin

DENATURING AGENTS: HOW TO SHIFT THE PROTEIN UNFOLDING
EQUILIBRIUM TO THE RIGHT
(5 pts)
Which of the following statements about the equilibrium “m” value, for the unfolding of a
biomolecule in the presence of a denaturing agent, is FALSE?
In your homework sheet, provide an answer to this question by simply writing a, b, c, d or e.
(a) The absolute value of the “m” value is proportional to the amount of solvent-accessible surface
is very different from the conformation of the heat-unfolded  repressor. (c) Cold unfolding has a Cp of zero. (d) Cold-unfolding is characterized by a negative H0 and S0. It is likely that the value of S0 is
negative mostly due to the reduced dynamics of the waters surrounding the cold-unfolded
protein. (e) Cold unfolding has a negative Cp.
6. DETERMINING HEAT CAPACITY CHANGES UPON PROTEIN UNFOLDING:
DIFFERENTIAL SCANNING CALORIMETRY (DSC).
(3 + 2 = 5 pts)
a. Draw the complete DSC plot (Cp vs T) for the equilibrium unfolding of a protein that is
sufficiently unstable so that both its cold- and heat-induced unfolding transitions can be observed in liquid solution by DSC. Be sure to draw lines, curves and areas with a biologically meaningful sign, based on what you know about protein folding and unfolding at equilibrium. Label Tm, Tm’, Cp,heat-unfolding and Cp,cold-unfolding on the plot.
b. Draw the DSC profile for the order-disorder transition of a nonpolar lipid with Cp = 0.
- 3 -
area that becomes exposed, upon denaturant-induced protein unfolding. (b) Proteins with a large and negative “m” value unfold easily in the presence of a denaturing
agent. (c) At the midpoint of a denaturant-induced equilibrium unfolding curve the “m” value switches
sign. (d) The “m” value is usually negative in the unfolding direction and positive in the folding
direction. The absolute value of “m” is the same in both directions. (e) The unfoldin

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