JR CHO
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'What one plate would you first inspect to conclude that the transformation occurred successfully? Why? What factors might influence transformation efficiency? Transformation efficiency" is the percentage of bacteria that took UP the plasmid: It does NOT just mean the number of bacteria that grew on the plate. Increasing the volume of bacteria that you plated on each plate would not increase the transformation efficiency. Explain the effect of each factor you mention'
Sri K.
Transformation Questions: 1. What was the purpose of the (+) Control? 2. What was the purpose of the (-) Control? 3. Calculate the transformation efficiencies for your group's data and the average data. Use the following information: a. The units of transformation efficiency are: number of transformants/µg of DNA. b. The final recovery volume of the cells was 0.11 ml c. The volume plated was 0.1ml d. The amount of DNA used is 25ng.
Jenny W.
11) What is meant by a control plate? What purpose does a control serve? Which plates are control plates in the lab? 12) If there are any genetically transformed bacterial cells, on which plate /plates would they most likely be located? Explain your predictions 13) Which plates should be compared to determine if any genetic transformation has occurred? Why?
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