Determine Km for GSK3.
The enzyme glycogen synthase kinase 3 (GSK3) plays a central role in Alzheimer's disease. The onset of Alzheimer's disease is accompanied by the production of highly phosphorylated forms of a protein referred to as *. GSK3 contributes to the hyperphosphorylation of such that inhibiting the activity of this enzyme represents a pathway for the development of an Alzheimer's drug. A compound known as Ro 31-8220 is a competitive inhibitor of GSK3. The following data were obtained for the rate of GSK3 activity in the presence and absence of Ro 31-8220 [A. Martinez et al., J. Medicinal Chemistry 45 (2002): 1292]:
Ro (Ms) [S] (M) [A] (M)
0 66.7 4.17 x 10^8
3.33 x 10^8 40.0 3.97 x 10^8
2.98 x 10^8 20.0 3.62 x 10^8
2.38 x 10^8 13.3 3.27 x 10^8
1.81 x 10^8 10.0 2.98 x 10^8
1.39 x 10^8 6.67 2.31 x 10^8
1.04 x 10^8
Express the Michaelis constant to three significant figures and include the appropriate units.
Value:
Units:
Submit
Request Answer
Part B
Determine Rmax for GSK3. Express the maximum rate to three significant figures and include the appropriate units.
Rmax:
Value:
Units:
Submit
Request Answer
Using the data with the inhibitor, determine Km. Express the Michaelis constant to three significant figures and include the appropriate units.
Km:
Value:
Units:
Submit
Request Answer
Using the data with the inhibitor, determine Rmax. Express the maximum rate to three significant figures and include the appropriate units.
Rmax:
Value:
Units: