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Hello students, the question of today's described the principle of flow cytometry, immunophenotyping and the role of gating and its importance.
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The principle of flow cytometry immunophenotyping involves the use of fluorescently labeled antibodies to detect specific cell surface markers and the cells are passed through a laser beam and the fluorescence emitted by the labeled antibodies is detected and analyzed.
01:26
Regarding the gating, which is the process of selecting a specific population of cells based on their fluorescence characteristics, allowing for the identification and analysis of specific cell types.
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Gating is important because it helps to eliminate background noise and focus on the cells of interest.
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Another question is regarding the pre -analytical errors.
02:46
So, if there are no pre -analytical errors and the instrument and assay are operating properly, a possible operator error might be the incorrect selection of cell markers or fluorophores, including the appearance of new population of stained cells that were not detected in previous assays.
03:47
Another question is regarding burkitt lymphoma and in burkitt lymphoma, cell markers such as cd19, cd20, cd22 and cd10 are typically expressed...