E. coli DNA polymerase III has a catalytic rate of 1000 nucleotides polymerized per second. E. coli genomic DNA consists of B-DNA, which has 10.4 base pairs per axial turn.
How fast does a template DNA strand spin at the E. coli replication fork? Express your answer in revolutions per second, or rps.
rate of spin =
In B-DNA, base pairs are spaced 3.4 Ā
apart along the helical axis. Note that 1 Ā
is equal to 1 × 10ā»ā“ μm.
What is the velocity of movement of the DNA polymerase III holoenzyme relative to the template? Express your answer in micrometers per second.
v =