Enzymes are immobilized on flat surfaces of plates in the form of monolayer (or very thin film) and placed in an agitated reactor for enzymatic reaction. The reactor is operated continuously. The bulk substrate concentration is So = 200 mg/l at steady state. When the reactor was operated at different agitation speeds, the following data were obtained:
RPM | kL (dm/h) | V (mg/dm²-h)
25 | 0.18 | 30
50 | 0.24 | 36
100 | 0.33 | 40
200 | 0.42 | 41.5
300 | 0.88 | 44
400 | 2.25 | 45
a) Determine surface concentration of the substrate for each RPM
b) Determine the kinetic constants for the enzymatic reaction
Km=22.62 mg/l, Vm=51.02 mg/dm²-h