Question

A generalized transduction experiment is set up so that a phage is carrying a donor strain with the genotype met+ bio+ thr+. This donor strain is used to transduce a recipient E. coli strain with the genotype met- bio- thr-. After transduction, the recipient population is plated on a minimal medium containing only organic salts and a simple carbon source supplemented with methionine and biotin. A large number of colonies are obtained. The wild-type genes indicate the bacteria do not need a supplemented minimal medium in order to grow. Any bacteria with mutant genes will require a supplement of methionine, biotin, or threonine to grow. The use of minimal medium without all the required nutrients is a type of marker selection. Marker selection utilizes the inability of some bacteria with mutant genotypes to grow on minimal medium by creating a medium that only select bacteria can grow on. Select the possible genotypes of the colonies that grew on this medium. met- bio+ thr- met- bio- thr+ met+ bio- thr+ met+ bio+ thr-

          A generalized transduction experiment is set up so that a phage is carrying a donor strain with the genotype met+ bio+ thr+. This donor strain is used to transduce a recipient E. coli strain with the genotype met- bio- thr-. After transduction, the recipient population is plated on a minimal medium containing only organic salts and a simple carbon source supplemented with methionine and biotin. A large number of colonies are obtained.

The wild-type genes indicate the bacteria do not need a supplemented minimal medium in order to grow. Any bacteria with mutant genes will require a supplement of methionine, biotin, or threonine to grow. The use of minimal medium without all the required nutrients is a type of marker selection. Marker selection utilizes the inability of some bacteria with mutant genotypes to grow on minimal medium by creating a medium that only select bacteria can grow on.

Select the possible genotypes of the colonies that grew on this medium.

met- bio+ thr-
met- bio- thr+
met+ bio- thr+
met+ bio+ thr-

A generalized transduction experiment is set up so that a phage is carrying a donor strain with the genotype met+ bio+ thr+. This donor strain is used to transduce a recipient E. coli strain with the genotype met- bio- thr-. After transduction, the recipient population is plated on a minimal medium containing only organic salts and a simple carbon source supplemented with methionine and biotin. A large number of colonies are obtained.

The wild-type genes indicate the bacteria do not need a supplemented minimal medium in order to grow. Any bacteria with mutant genes will require a supplement of methionine, biotin, or threonine to grow. The use of minimal medium without all the required nutrients is a type of marker selection. Marker selection utilizes the inability of some bacteria with mutant genotypes to grow on minimal medium by creating a medium that only select bacteria can grow on.

Select the possible genotypes of the colonies that grew on this medium.

met- bio+ thr-
met- bio- thr+
met+ bio- thr+
met+ bio+ thr-

Added by Kayla T.

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