00:01
Hello students, in this question it is talking about restriction, restriction mapping.
00:06
It is giving us a plasmid and different restriction enzyme digestion fragments of bam h1, eco r1, nd1 and their combinations.
00:18
These are the following fragments given to us.
00:21
The total size is 12 kb.
00:25
If we add up the fragments, it will give us the value of 12 kb in all the cases.
00:32
It is common.
00:33
Next it is asking us to show the banding pattern in a gel electrophoresis.
00:40
Since there are four fragments, highest number is four fragments, we will put four lanes and the sizes we will differentiate based from the size 1 kb to 11 kb.
00:57
The highest fragment is 10 .6 in size and the lowest one is 0 .5 in size.
01:05
Therefore, we will put the fragments based on the size starting from the first one that is bam h1, two fragments, one is at 1 .4 and another is at 10 .6.
01:25
We will put it here.
01:27
In this way, we will put all the fragments.
01:30
In the next part of the question, we are asked to draw the restriction map of this plasmid, show the restriction enzyme locations...