High-performance liquid chromatography (HPLC) is an important analytical instrument for mixture analysis. The chromatogram was obtained by analyzing a multi-component mixture using a C-18 reversed phase HPLC column. The mobile phase was 35% acetonitrile in water.
Chromatogram A:
After optimizing the elution conditions using a binary mixture of water and acetonitrile, chromatogram B was obtained.
Chromatogram B:
What type of liquid chromatography was used in the above analysis?
b) With reference to the elution conditions in obtaining Chromatogram A, suggest a stronger elution solvent composition based on a binary mixture of water and acetonitrile. Explain the notion behind your suggestion.
c) Define "gradient elution." Which of the above chromatograms was obtained from gradient elution?
d) Suggest an elution scheme for Chromatogram B and briefly explain the notion behind your suggestion.
e) Explain why the intensity of peak 8 is so much higher in Chromatogram B than in Chromatogram A.