00:01
Hello students, let's begin with this question.
00:03
So here we have to write down the tlc effect.
00:05
The first one, students who loaded his sample onto the column diluted with too much of solvent.
00:09
So yes, yes, this will affect the tlc result.
00:21
So here, in this case, using too much of solvent, this will cause the compound to spread out more on the column and leads to the poor separation and also result in the overlapping of the spots.
01:07
Now, the next one is students who used too non -polar and eluent system when running through the column.
01:13
So yes, this affects the tlc results.
01:21
So here, using the non -polar eluent system, this will not effectively separate the polar components, compounds and this will leads to the poor separation.
01:54
Next one is the students who used too polar and eluent system when running the column.
02:00
So no, this does not affect the tlc results.
02:12
This is so because here the polar eluent system, this will cause the compounds to move too quickly through the column and this will leads to the better separation...