3. (i) A particle has a terminal velocity in a centrifuge of 40 ?m/s at an acceleration (a) of 10? x g. What is its sedimentation coefficient in Svedbergs (S). (ii) Calculate the angular velocity (?) of the particle in the above question, where the acceleration is 10? x g, in a rotor of radius (r) 25 cm. (iii) If the particle has a mass of 10?¹²g How far will it move in 10 minutes under these conditions? (iv) Complete the data in the Table below; what organelle is enriched after differential centrifugation of homogenate.
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8 m/s^2 = 1038.8 m/s^2 Substitute the values into the formula: S = 40 * 10^-6 / 1038.8 S = 3.85 * 10^-14 Svedbergs Show more…
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