00:01
First we need to determine what the factor is between concentration of the analyte and the standard.
00:08
So we'll refer to this as f and we can calculate it a couple different ways as long as we follow the same pattern.
00:18
We'll take a ratio of the concentration of the analyte to the standard.
00:24
That being 5 .32 mmol for the analyte to 2 .36 mmol for the standard.
00:37
We then set that equal to f multiplied by the ratio of the signals.
00:43
They tell us that the peak area, a solution containing 5 .23 mmol analyte yielded a peak area that was twice that of the standard.
00:57
So it's going to be 2 over 1.
01:02
So now we can solve for this f value.
01:05
F is going to be 1 .127.
01:12
Which means the analyte signal is 1 .127 times that of the standard at the same concentration.
01:23
So now knowing f we can then calculate the concentration of the unknown in the original solution.
01:33
The concentration of the standard after dilution divided by the concentration of the analyte after dilution, which we'll call x, divided by the concentration of the standard after dilution.
01:51
We took 1 ml of 6 .93 ml.
01:56
We added it to 5 ml of unknown and then diluted to 10 ml...