Question

In general, native proteins (N state) are in equilibrium with the denatured form (U state) such that: Protein (N) ⇌ Protein (U) Ribonuclease protein catalyzes the degradation of RNA into smaller components. The following temperature-dependent concentrations of the N and U forms of ribonuclease protein were experimentally determined, under equilibrium, for a total protein concentration of 1.0 x 10^-3 mol/L Temp (K) N (mol/L) U (mol/L) 323 9.97 x 10^-4 2.57 x 10^-6 373 8.6 x 10^-4 1.4 x 10^-4 Determine the standard enthalpy change (ΔrHo) of the denaturation reaction of the Ribonuclease protein; assuming that ΔrHo is temperature independent within this T-range [R = 8.314 J.mol^-1.K^-1]. Based on your calculations, which one of the following statements is correct:

          In general, native proteins (N state) are in equilibrium with the denatured form (U state) such that:
Protein (N) ⇌ Protein (U)
Ribonuclease protein catalyzes the degradation of RNA into smaller components. The following temperature-dependent concentrations of the N and U forms of ribonuclease protein were experimentally determined, under equilibrium, for a total protein concentration of 1.0 x 10^-3 mol/L

Temp (K)   N (mol/L)   U (mol/L)
323           9.97 x 10^-4           2.57 x 10^-6
373           8.6 x 10^-4             1.4 x 10^-4

Determine the standard enthalpy change (ΔrHo) of the denaturation reaction of the Ribonuclease protein; assuming that ΔrHo is temperature independent within this T-range [R = 8.314 J.mol^-1.K^-1]. Based on your calculations, which one of the following statements is correct:

Added by Anthony G.

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