Is DNA soluble in ethanol? Explain your answer. 4. Is DNA soluble in ethanol? Explain your answer.
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You isolated genomic DNA from your favorite bacterium and since you are in hurry you ask your lab mate whether she thinks it is a good idea to skip the drying step after ethanol precipitation and before you add the TE buffer: She is rather experienced and will most likely answer sure go ahead; a little ethanol is not going to hurt not a good idea, if the final ethanol concentration is too high; the DNA is not going into solution I have no idea, but maybe you should add more EDTA if you do that just add a larger volume of TE and you will be fine
Emily H.
Madhur L.
What is the role of alcohol in extracting DNA? 1. DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3. DNA is a nonpolar molecule with an overall negative charge, and as such is soluble in alcohol, and therefore precipitates. 4. DNA is a polar molecule with an overall positive charge, and as such is soluble in alcohol, and therefore precipitates.
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