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Make me a phosphate buffer with a given pH value (see below) so I can study some blood proteins. The concentration of ions in blood (its ionic strength) is in the neighborhood of 150 mM. Make me enough so that I can have at least 1000 samples of 2 mL each. Data: pKa values for phosphoric acid, H3PO4, are 2.1, 7.2, and 12.3. You have three bottles of crystalline solids: NaH2PO4, mw 120 g mol^-1; Na2HPO4, mw 142 g mol^-1; NaPO4 mw 164 g mol^-1. I spent the big bucks on anhydrous salts for you. Produce a Standard Operating Procedure (SOP) for mixing the biological buffer. Imagine that your lab regularly needs to make these batches of phosphate buffer. The pH of the buffer solution is to be equal to the following value. Take the last digit of your student's number (8) and divide it by ten. Then add that to 6.55. The total concentration includes all practical information for your method.

          Make me a phosphate buffer with a given pH value (see below) so I can study some blood proteins. The concentration of ions in blood (its ionic strength) is in the neighborhood of 150 mM. Make me enough so that I can have at least 1000 samples of 2 mL each. Data: pKa values for phosphoric acid, H3PO4, are 2.1, 7.2, and 12.3. You have three bottles of crystalline solids: NaH2PO4, mw 120 g mol^-1; Na2HPO4, mw 142 g mol^-1; NaPO4 mw 164 g mol^-1. I spent the big bucks on anhydrous salts for you. Produce a Standard Operating Procedure (SOP) for mixing the biological buffer. Imagine that your lab regularly needs to make these batches of phosphate buffer. The pH of the buffer solution is to be equal to the following value. Take the last digit of your student's number (8) and divide it by ten. Then add that to 6.55. The total concentration includes all practical information for your method.

Added by Jose Francisco C.

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