Question

Mixtures of amino acids can be analyzed by separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3-) groups flow down the column at different rates because of two factors that influence their movement: ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer. Amino acid pair | Elutes first | Elutes second Asp and Arg | Glu | Arg Lys and Met Gly and Glu Ala and Leu Trp and Ser Answer Bank Ala, Gly, Leu, Lys, Trp, Met, Asp, Ser

          Mixtures of amino acids can be analyzed by separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3-) groups flow down the column at different rates because of two factors that influence their movement: ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin.

For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer.

Amino acid pair | Elutes first | Elutes second
Asp and Arg | Glu | Arg
Lys and Met
Gly and Glu
Ala and Leu
Trp and Ser

Answer Bank
Ala, Gly, Leu, Lys, Trp, Met, Asp, Ser

Mixtures of amino acids can be analyzed by separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3-) groups flow down the column at different rates because of two factors that influence their movement: ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin.

For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer.

Amino acid pair | Elutes first | Elutes second
Asp and Arg | Glu | Arg
Lys and Met
Gly and Glu
Ala and Leu
Trp and Ser

Answer Bank
Ala, Gly, Leu, Lys, Trp, Met, Asp, Ser

Added by Mireia O.

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