0:00
All right.
00:01
So our question is we have normal dna and mutated dna, and we need to know what the mrna code is for the mutated dna, what the amino acid sequence is going to be, where the mutation happened between the two dna strands, on what type of mutation and if the protein is still going to function.
00:18
So the first one i'm going to start off with is where the mutation happened.
00:26
So if we look at this fourth codon, we see in the normal it's cgc.
00:32
In the mutated, it's cgg.
00:35
So we see that last sequence or that last base was changed from a c to a g.
00:43
So that's where the mutation happened.
00:46
How we get our mrna code is we have to use our base pair rule.
00:51
For mrna, c's become g's.
00:54
So our first one is going to be g -c -c.
00:59
And then instead of t's, we're going to have u's and a's.
01:02
So a becomes a u and t becomes an a.
01:08
So for our second one, we have a -t -a, third one, a -u -g.
01:12
Next one, g -c -c -a -a -u -c -g, a -c -g -g -u -c -g -u -c -g -u -c -g.
01:31
Ccc, ccc, gg.
01:37
One thing also, the reason we're using these two dna strands instead of the other one is because n rna polymerase reads dna from 3 prime to 5 prime.
01:49
So that's why we're using these two sequences.
01:53
How we get our amino acid sequences now is we use the mrna and we use this chart here to figure out.
02:03
The amino acid sequence.
02:04
So we're using the codon.
02:06
And then we'll get our three -letter abbreviation for the amino acid.
02:10
So for our first one, we have gcc.
02:13
So we'll use a different color.
02:20
So we have g is the first letter.
02:22
Then we move in.
02:23
Second letter is a c.
02:26
The third letter is a c.
02:28
So we have our first amino acid is a -l -a.
02:33
However, normally we start with our start codon, which is going to be this met right here.
02:48
But we can just keep going until we get to met just for some extra practice.
02:51
So we have u -a -u is our next one.
02:54
So u -a -u is tier.
03:06
And then we have aug, which as we just showed, aug is our start codon...