Question

Off-Week Activity 2 – TLC Review Activity (10): Watch the presentation and helpful videos on thin-layer chromatography and answer the questions below. 1. (2) Rank the following compounds from most (1) to least (4) polar. (HINT: Refer to Appendix 3 in your lab manual) H3C-C(=O)OH A (acetic acid) Hexagon with Br B (bromohexane) Hexagon with circle C (benzene) CH3CH2-O- Na+ D (sodium ethoxide) 2. (4) Using the letters A-B-C-D, assign the molecules shown in question #1 above to their appropriate positions (spots) on the TLC plate drawn below, considering that you used a silica gel (stationary phase) plate and methylene chloride as the developing solvent (mobile phase). 62 mm solvent front 37 mm 28 mm 8.7 mm 1.9 mm baseline methylene chloride (CH2ClCH2Cl) molecule/spot identity 3. (4) Calculate the Rf values for molecules A-B-C-D shown on the plate above. Show your work, with units, and circle your answers. cmpd A: cmpd B: cmpd C: cmpd D:

          Off-Week Activity 2 – TLC Review
Activity (10): Watch the presentation and helpful videos on thin-layer chromatography and answer the questions below.
1. (2) Rank the following compounds from most (1) to least (4) polar. (HINT: Refer to Appendix 3 in your lab manual)
H3C-C(=O)OH A (acetic acid)
Hexagon with Br B (bromohexane)
Hexagon with circle C (benzene)
CH3CH2-O- Na+ D (sodium ethoxide)
2. (4) Using the letters A-B-C-D, assign the molecules shown in question #1 above to their appropriate positions (spots) on the TLC plate drawn below, considering that you used a silica gel (stationary phase) plate and methylene chloride as the developing solvent (mobile phase).
62 mm solvent front
37 mm
28 mm
8.7 mm
1.9 mm
baseline
methylene chloride (CH2ClCH2Cl)
molecule/spot identity
3. (4) Calculate the Rf values for molecules A-B-C-D shown on the plate above. Show your work, with units, and circle your answers.
cmpd A:
cmpd B:
cmpd C:
cmpd D:

Off-Week Activity 2 – TLC Review
Activity (10): Watch the presentation and helpful videos on thin-layer chromatography and answer the questions below.
1. (2) Rank the following compounds from most (1) to least (4) polar. (HINT: Refer to Appendix 3 in your lab manual)
H3C-C(=O)OH A (acetic acid)
Hexagon with Br B (bromohexane)
Hexagon with circle C (benzene)
CH3CH2-O- Na+ D (sodium ethoxide)
2. (4) Using the letters A-B-C-D, assign the molecules shown in question #1 above to their appropriate positions (spots) on the TLC plate drawn below, considering that you used a silica gel (stationary phase) plate and methylene chloride as the developing solvent (mobile phase).
62 mm solvent front
37 mm
28 mm
8.7 mm
1.9 mm
baseline
methylene chloride (CH2ClCH2Cl)
molecule/spot identity
3. (4) Calculate the Rf values for molecules A-B-C-D shown on the plate above. Show your work, with units, and circle your answers.
cmpd A:
cmpd B:
cmpd C:
cmpd D:

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