00:01
Hello students, we will discuss a problem of biochemistry and we will discuss the molecular weight of two proteins that is 30 ,000 da and 92 ,000 da has been given to us.
00:12
Their relative electrophoretic mobility is also mentioned and we have to calculate the apparent mass of the unknown protein.
00:20
We can start this problem by applying certain formulas for that we have to write down the data once again.
00:30
And the data is for the first protein, the molecular weight is equal to 30 ,000 da.
00:38
Da is a unit that is used to calculate the molecular weight of the very heavy polymers and especially in the case of natural polymers, for example, protein.
00:49
It is abbreviation of its dutton and mobility of this protein has been given as 0 .8.
01:03
And if we write log molecular weight that will be equal to log of 30 ,000 da and that can also be written as log of 30 kda, where kilodalton is the bigger unit of the same molecular weight and this value is equal to 4 .477.
01:44
After this we can write the equation for the straight line and y is equal to mx plus c and while putting our data as we can write 4 .477 is equal to m and instead of x we have to write the mobility value that is 0 .8 and plus c and let's say this is the first equation or the a equation for the second protein, similarly, we can write and that log molecular weight can be written as log of 92 kda and this value is equal to 4 .964.
02:41
And if we write the same equation for it, y is equal to mx plus c, that can be written as 4 .964 is equal to m and its mobility is given as 0 .41 plus c and let's say this is the second equation...