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QUESTION 3 An Azotobacter vinelandii strain is cultured in a 15m^(3) stirred fermenter for alginate production. Under current operating conditions, k_(La) is 0.17s^(-1). The solubility of oxygen in the broth is approximately 8\times 10^(-3)k(g)/(m^(3)). 3.1 The specific rate of oxygen uptake is 12.5mmo(l)/(g).h. What is the maximum cell concentration supported by oxygen transfer in the fermenter? 3.2The bacteria suffer growth inhibition after copper sulphate is accidentally added to the fermentation broth just after the start of the culture. This causes a reduction in the oxygen uptake rate to 5mmo(l)/(g).h. What maximum cell concentration can now be supported by oxygen transfer in the fermenter?

          QUESTION 3
An Azotobacter vinelandii strain is cultured in a 15m^(3) stirred fermenter for alginate
production. Under current operating conditions, k_(La) is 0.17s^(-1). The solubility of
oxygen in the broth is approximately 8\times 10^(-3)k(g)/(m^(3)).
3.1 The specific rate of oxygen uptake is 12.5mmo(l)/(g).h. What is the maximum cell
concentration supported by oxygen transfer in the fermenter?
3.2The bacteria suffer growth inhibition after copper sulphate is accidentally added to
the fermentation broth just after the start of the culture. This causes a reduction in
the oxygen uptake rate to 5mmo(l)/(g).h. What maximum cell concentration can now
be supported by oxygen transfer in the fermenter?

Added by Luis Miguel B.

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