QUESTION 19 When operating a centrifuge, you must ensure that it is with another test-tube of
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When you use a microcentrifuge what must you always do? (select all that apply!) Group of answer choices Make sure the centrifuge is balanced Have someone supervise you Always have an even number of tubes Make sure the tubes have the same amount of volume in them
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Centrifugation. Certain procedures necessitate precise centrifugation conditions, which must be specified in terms of relative centrifugal force (RCF) expressed in units of gravity (times gravity or × g). Many microcentrifuges only have settings for speed (revolutions per minute, RPM), not relative centrifugal force. Consequently, a conversion is required to ensure that the appropriate setting is used in an experiment (Thermo Scientific). Rotor Centrifuge tube r = 5cm r = 10cm Centrifuge tube Shaft (axis) Shaft (axis) a. Calculate the RPM for the given two centrifuges with rotors with indicated spinning radii that needs a relative centrifugal force of 5,000 g. b. You are transferring the parameters from a centrifuge of radius 5 cm at a speed of 2,000 RPM to another centrifuge of radius 10 cm. At what speed should you set the new centrifuge?
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