00:01
Hello student in this question we will be talking about chromatography this is a technique to separate the amino acids amino acids which is the building block of proteins by their charge by their mass and many more things and to make the separation based on that reference value we can also find out the unknown sample by a rf value which can be calculated as the distance travel by travel by amino acid spot and this will be divided by the distance travel by the solvent so distance by solvent so here for this question this particular chart we have to fill in the blanks by the given informations so the amino acids in the chromatographic paper will be colorless so this is colorless and for all the thing the amino acids will be colorless.
01:33
So i am not repeating this again and again.
01:36
The distance traveled by the solute is 0 .69 and this solute will be traveled for each of the amino acids.
01:45
So these values will be 0 .69, 0 .69, 0 .69, 0 .69, 0 .69, 0 .69, 0 .69 and 0 .69 .69.
01:58
Now, we have to calculate the distance traveled by the amino acid, which will help us to give the rf value.
02:08
So, the distance traveled by phenylalanine is 0 .47.
02:14
That of the alonine is, that of the alonine is 0 .26.
02:18
Cestine is 0 .1794.
02:23
That for serine is 0 .19.
02:28
That for lysine is 0 .09.
02:31
For aspartic acid, it is 0 .16.
02:34
And for unknown, it is 0 .42.
02:38
Now the rf value will be distance travel by the amino acid divided by distance travel by the salt...