Question

Researchers discover an enzyme that catalyzes the reaction A \leftrightarrow B. They find that the $K_m$ for the substrate A is 4 µM, and the $k_{cat}$ is 20 min$^{-1}$. In an experiment [A] = 6 mM and $V_0$ = 480 nM/min. The [E] used in this experiment is A) 0.024 nM B) 24 nM C) None of the options are correct D) 40 nM E) 400 nM

          Researchers discover an enzyme that catalyzes the reaction A \leftrightarrow B. They find that the $K_m$ for the substrate A is 4 µM, and the $k_{cat}$ is 20 min$^{-1}$. In an experiment [A] = 6 mM and $V_0$ = 480 nM/min. The [E] used in this experiment is
A) 0.024 nM
B) 24 nM
C) None of the options are correct
D) 40 nM
E) 400 nM

Researchers discover an enzyme that catalyzes the reaction A ↔B. They find that the Km for the substrate A is 4 µM, and the kcat is 20 min^-1. In an experiment [A] = 6 mM and V0 = 480 nM/min. The [E] used in this experiment is
A) 0.024 nM
B) 24 nM
C) None of the options are correct
D) 40 nM
E) 400 nM

Added by Melissa A.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Sri K

03/23/2023

Step 1

Since Vmax = kcat * [E], we can rearrange the equation to solve for [E]. Show more…

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Researchers discover an enzyme that catalyzes the reaction AB. They find that the Km for the substrate A is 4 M and the kcat is 20 min^(-1) in an experiment [A] = 6 mM and Vo = 480 nM/min. The [E] used in this experiment is A) 0.024 nM B) 24 nM C) None of the options are correct D) 40 nM E) 400 nM.