Question

Screening of RNAs by SELEX The practical limit for the number of different RNA sequences that can be screened in a SELEX experiment is $10^{15}$. (a) Suppose you are working with oligonucleotides 32 nucleotides long. How many sequences exist in a randomized pool containing every sequence possible? (b) What percentage of these can be screened in a SELEX experiment? (c) Suppose you wish to select an RNA molecule that catalyzes the hydrolysis of a particular ester. From what you know about catalysis, propose a SELEX strategy that might allow you to select the appropriate catalyst.

          Screening of RNAs by SELEX The practical limit for the number of different RNA sequences that can be screened in a SELEX experiment is $10^{15}$. (a) Suppose you are working with oligonucleotides 32 nucleotides long. How many sequences exist in a randomized pool containing every sequence possible? (b) What percentage of these can be screened in a SELEX experiment? (c) Suppose you wish to select an RNA molecule that catalyzes the hydrolysis of a particular ester. From what you know about catalysis, propose a SELEX strategy that might allow you to select the appropriate catalyst.

Added by Sharon H.

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