se-Van Deemter equation to answer the questions below: In mobile phase C17 at 175°C k=4.95 25m x 0.25mm Crosslinked trimethyl silicone
N2
1.0- (mm) E.T.P 0.6 I
(4.
He
H2
0.4
0.2-
10
20 30 40 50 60 70 Average Linear Velocity (cm/sec.)
80
90
se-Van Deemter equation to answer the questions below:
a) What is the optimal flow rate for the best separation of solutes with He mobile phase?