00:01
So let's start from beginning.
00:02
So first of all, you're going to type in the first ncbi .nlm .n .h .g .g .g.
00:08
That will bring you to this national library of lab medicine, the ncbi website, which is the biggest database for all sorts of literature and resources that we need to study our bio and biochemistry field.
00:25
So first of all, you will go ahead and then click from all database.
00:31
And then go all the way to nucleotide.
00:37
All right.
00:38
And then you will copy and paste the number in kj 660 -346 .2 offered by the question.
00:59
So during this process, this accession number will be used to search the database.
01:04
And then the next page shows you the information of this organization.
01:08
As you can see, to answer a question, the sequence that you're looking at right now is a very ebola virus isolated from human, i guess, homo sapien, which is a human, it's a wild type.
01:25
So it's a complete genome of ebola virus.
01:28
So the size of this complete genome is 18 ,959 .8.
01:39
So that's the size of the complete genome.
01:44
So what else if, what other information can you get from this nucleotized sequence? well, first of all, you can look at the crna right here.
01:56
This tells you that the genome of this ebola virus is actually made of rna.
02:02
Crna stands for complementary rna, but the synthetic rna that is reverse transcribed from a single dna strand because this virus is isolated from homo sapiens.
02:16
So the sequence is deduced by the sequence that is isolated.
02:22
So first information that you can get from this page is the ebola virus is made of rna.
02:29
Some other information you can get from page if you pull down, it tells you the references that would actually identify this genome of the virus.
02:39
And then if you go down, and then you can see that's the more information or detailed information about this genome.
02:48
All right.
02:48
And then it tells you certain mrna sequence, what gene it encodes for.
02:55
And then you can tell the cds, these are the coding regions.
02:58
That means certain sequence, these are mrna sequence.
03:02
They will be coded into a certain protein, and then it will show you the protein sequence right here.
03:06
Every single letter here is a protein amino acid sequence run right here.
03:14
So you can tell that, for example, in this case, it's a piece of gene, and then that's a gene called np, and the beginning and end of mrna sequence of this being.
03:27
And also the cds tells you if we translate the mrna sequence into a protein sequence, this is the most likely translation product.
03:37
Of the mrna sequence.
03:39
So you can see there's multiple genes involved, and it tells you the beginning and of the sequence, and then it tells you what the gene is, and then again show you all the coding sequence of that particular gene.
03:56
Now, if you go down all the way, it tells you the gene bank format of the entire genome.
04:04
As you can see, these every single one, the single nucleotide of, the genome and it tells you, these numbers tells you the corresponding number of nucleotidid within the sequence.
04:19
So you can see it's a very long 15 ,000 base pair sequence.
04:23
It's very long.
04:24
So you can use these sequence to do any bioinformatics processing in the future.
04:31
All right, now let's move on to the next question and i ask you, if you scroll down and for this on this page and click on the blue link labeled as mrrna and look for gene gp.
04:46
All right.
04:47
So we will still go back to the beginning of the page and then we here, scroll down, and you will see gene, this is np and then you can go down.
04:58
Bp 40, keep going.
05:02
Okay, now we got it.
05:03
So this is a gene that's called gp right here, right? and then this mrna, the blue link, will show you the m rna mnna mna sequence of this is q &e, p.
05:16
So we are going to put this mrna.
05:21
Now you can tell that this whole brown sequence is highlighted.
05:27
So that means the gp gene is actually, this is where the gp gene is within the sequence.
05:34
You can see this is star from somewhere here, and it tells you where it begins.
05:42
It begins from 5 ,900 and it's going to be over by 8 ,305.
05:52
So this is where it begins, 5905 .00 and then this is where it ends.
05:59
All right, so we are going to show this in a fasta format, which you are going to click down there, fasta.
06:11
All right, now, the next page you will see is the faster sequence of this particular gene -gp.
06:20
So this gene, this fragment, or i guess the dna sequence, is the sequence of gpg.
06:31
So now the question asks you to give the first 10 nucleotide of the sequence.
06:37
So i guess the first nucleotide will be found here.
06:40
The g1, 2, 3, 4, 5, 6.
06:44
7, 8, 9, 10.
06:48
So the first 10 nucleotite will be g -a -t, g -a -a -g -a -g -a -t -c.
06:54
That will be the first technically type.
06:58
All right...