A solvent passes through a chromatography column in 2.42 min, but the solute requires 9.47 min. What is the retention factor, k? k = 2.91 What fraction of the time does the solute spend in the mobile phase in the column? tfraction in mobile phase = .255 The volume of the stationary phase is 0.162 times the volume of the mobile phase in the column (Vs = 0.162Vm). What is the partition coefficient, K, for this system? K = 12.34
Added by Francisca N.
Close
Step 1
So, k = (tr - t0) / t0 = (9.47 min - 2.42 min) / 2.42 min = 2.91. Second, the fraction of the time the solute spends in the mobile phase is calculated by dividing the time it takes for the solvent to pass through the column (t0) by the time it takes for the Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 72 other Chemistry 101 educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Calculate the partition coefficient for the analyte when an unretained solute passes through a chromatography column in 3.7 min and the analyte requires 8.4 min. Assume the volume of the mobile phase is 1.4 times the volume of the stationary phase.
Md.Daniyal A.
4. Chromatographic separations. A typical chromatography column is 15 cm long, has a phase ratio ϕ = 0.2, and has a mobile phase velocity of 2 mm s⁻¹. (a) What is the velocity of a solute that has a partition coefficient K = 20 for binding to the stationary phase? (b) Suppose you can distinguish two peaks as close as 2 mm together when they reach the end of the column. If one peak corresponds to K = 20, what is the partition coefficient for the other peak?
Shaiju T.
Recommended Textbooks
Chemistry: Structure and Properties
Chemistry The Central Science
Chemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD