Spinach column chromatography 1. Did the compounds in the mixture elute from the column in most to least polar or vice-versa? Provide an explanation. 2. What was the purpose of the sand BOTH times it was used in the experiment? Be brief. 3. Describe any colored bands you may have seen, and when they occurred, in today's lab. 4. How effective were the separations during the TLC analysis (following the separations in the column)?
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This means that polar compounds will be retained in the column, while non-polar compounds will elute first. Therefore, in spinach column chromatography, the compounds in the mixture would elute from the column from least polar to most polar. Show more…
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