Question

Texts: A. Buffer Preparation - Prepare 10 mL of the following buffers by diluting the stock solutions in H2O (listed below): 1) Hypotonic lysis buffer: - 5 mM HEPES [pH 7.9] - 1.5 mM MgCl2 - 10 mM KCl - 1 mM beta-mercaptoethanol (BME) 2) Nuclear Extraction Buffer: - 20 mM HEPES [pH 7.9] - 25% glycerol - 1 M NaCl - 1.5 mM MgCl2 - 0.5 mM EDTA - 1 mM BME 3) SDS Lysis Buffer: - 500 mM NaCl - 1% SDS - 1 mM BME Stock Solutions: - 5 M NaCl - 1 M HEPES [pH 7.9] - 750 mM MgCl2 - 1 M KCl - 1 M BME - 50% glycerol - 100 mM EDTA - 10% SDS Please complete all dilution calculations before class. The calculations will be evaluated as part of the pre-lab.

          Texts: 

A. Buffer Preparation - Prepare 10 mL of the following buffers by diluting the stock solutions in H2O (listed below):

1) Hypotonic lysis buffer:
- 5 mM HEPES [pH 7.9]
- 1.5 mM MgCl2
- 10 mM KCl
- 1 mM beta-mercaptoethanol (BME)

2) Nuclear Extraction Buffer:
- 20 mM HEPES [pH 7.9]
- 25% glycerol
- 1 M NaCl
- 1.5 mM MgCl2
- 0.5 mM EDTA
- 1 mM BME

3) SDS Lysis Buffer:
- 500 mM NaCl
- 1% SDS
- 1 mM BME

Stock Solutions:
- 5 M NaCl
- 1 M HEPES [pH 7.9]
- 750 mM MgCl2
- 1 M KCl
- 1 M BME
- 50% glycerol
- 100 mM EDTA
- 10% SDS

*Please complete all dilution calculations before class. The calculations will be evaluated as part of the pre-lab.*

Added by Gonzalo Y.

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